Cyclospora cayetanensis is a coccidian protozoan parasite causing cyclosporiasis, a diarrheal disease worldwide, associated with the consumption of fresh produce or water contaminated with the sporulated oocysts from the parasite. This parasite can only infect human hosts, with oocysts that have been sporulated in the environment within 1-14 days and then ingested. Fresh produce has been reported to be contaminated with C. cayetanensis in endemic areas of several countries and recently has been identified in produce grown in the USA. Foodborne outbreaks in the U.S. caused by C. cayetanensis associated with fresh produce have been recurring almost every year since 2000 (CDC). In 2021, two major multistate outbreaks were reported in the United States in which various people reported eating various types of leafy greens; the source of the outbreak was not identified. In total in 2021, there were 1,020 illnesses reported and 70 hospitalizations (CDC). In 2022 there were 1,129 illnesses and 74 hospitalizations, and 2023 has had 1,063 illnesses and 79 hospitalizations (CDC). A better understanding of the regional prevalence, occurrence, and how fresh produce becomes contaminated with Cyclospora is essential to prevent cyclosporiasis infections and outbreaks.